COMPARATIVE HYBRIDIZATION OF MITOCHONDRIAL AND CYTOPLASMIC AMINOACYL TRANSFER RNA WITH MITOCHONDRIAL DNA FROM RAT LIVER

Differences between mitochondrial and cytoplasmic transfer RNA and aminoacyl transfer RNA synthetases from rat liver.

The mitochondria of yeast,1 Neurospora,2 and Tetrahymena3 have been shown to contain transfer RNA (tRNA). Barnett et al.4 have found, in addition, that Neurospora mitochondria contain 15 aminoacyl-tRNA synthetases, at least three of which acylate only mitochondrial tRNA. The purpose of this communication is twofold. First we will present evidence showing that, as with eukaryotic microbial syste...

Mitochondrial-specific aminoacyl-RNA synthetases.

Numerous lines of evidencel'indicate that protein synthesis occurs within mitochondria, and tRNA8, 15, 16 and aminoacyl-RNA synthetases8 have been prepared from these organelles. More recently, two very interesting aspects of mitochondrial protein synthesis have been described: (a) Although amino acid incorporation by yeast cytoplasmic ribosomal systems is not inhibited by chloramphenicol,'7 pr...

Fluorescent in situ hybridization of mitochondrial DNA and RNA.

To reveal nucleic acid localization in mitochondria, we designed molecular beacon fluorescent probes against: i) the light strand complementary to ND5 mitochondrial DNA (mtDNA) gene (annealing also to corresponding mRNA); ii) displacement (D) loop 7S DNA (annealing also to parallel heavy strand mtDNA and corresponding light strand transcript); iii) the proximal D-loop heavy strand displaced by ...

Partial purification of mitochondrial RNA polymerase from rat liver.

Mitochondrial RNA polymerase activity from rat liver has previously been demonstrated in intact organelles. This activity has now been solubilized, partially purified, and shown to be a true polymerase, free of nuclease. The enzyme is derived from mitochondria and is not from contaminating bacteria or nuclear components. The enzyme is distinguished from its nuclear counterparts by its behavior ...

More evidence for a particle character of aminoacyl-transfer RNA synthetases isolated from rat liver.